N-Glycans: Phenotypic Homology and Structural Differences between Myocardial Cells and Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Figure 10
Rarely expressed NeuGc-containing glycans in iPSCs and iPSC-CMs.
(a) Relative quantities of NeuAc- and NeuGc-containing glycans; Monosialoglycans containing NeuAc and Disialoglycans containing two NeuAc (A, A/A): the total volume of M1, M2-1, M2-2, M5-M8, M10-M14, M16-M19, M20-2, M21-M23, D4-1, D4-2, D6, D7, D9, D10-1, D12, Disialoglycan containing NeuAc and NeuGc (A/G): D11, Monosialoglycan containing NeuGc and Disialoglycan containing two NeuGc (G, G/G): the total volume of M2-3, M3, M4, M9, M15, M20-1, D1-D3, D5-1, D5-2, D8, D10-2. (b) Transcript expression of ST3Gal-III, ST3Gal-IV, ST6Gal-I, and CMAH; Transcript expression of glycosyltransferases in iPSCs, iPSC-CM, and heart cells was analyzed by real-time PCR. Results are expressed as the mean ± standard deviation. *P<0.05 vs. Heart, †P<0.05 vs. iPSC-CM (all of the 959A2-1 CM, 959C1-1 CM and 956F-1 CM).