Development, Characterization and Application of Monoclonal Antibodies against Brazilian Dengue Virus Isolates
Figure 3
Cross-reactivity of mAbs D3 424/8G and D3 863/G7/H7 against WNV, SLEV and YFV.
Vero E6 cells were infected with WNV (A), whereas Huh7.5 cells were infected with YFV and SLEV (B). Cells were fixed in methanol:acetone and stained with mAbs, followed by Alexa-Fluor 488-conjugated anti-mouse immunoglobulin. Monoclonal antibody 4G2 and a non-correlated anti-hantavirus mAb (572/7A) were used as positive and negative controls, respectively. Images were obtained with a Leica AF6000 Modular System. Scale bars are 30 µm.