Prosaposin Overexpression following Kainic Acid-Induced Neurotoxicity
Figure 5
a, b: Double immunofluorescence light micrographs of the rat hippocampal CA1 regions of normal control (a) and animals 3 days after KA injection (b), stained with anti-PS and anti-Tau.
The rectangles 1–6 in figures (a) and (b) are shown at a higher magnification in the lower column. Nuclei are stained with DAPI (blue), PS stained with anti-PS (1–6R: red), axons and axon terminals with anti-Tau (1–6G: green) and merged images (1–6M). Arrows indicate the double-stained axon terminals containing PS. c–f: Double immunofluorescence light micrographs of Tau-positive axons in the CA1 regions of normal control (c) and experimental animals 3 days after KA injection (e), stained with anti-PS and anti-Tau. Panels d and f are black-and-white images of the PS-IR shown in c and e, respectively, for the NIH Image analysis (g). Note that the PS-IR granules (arrowheads) in the Tau-positive axons in the KA-injected animals are larger than those in the control. g: The ratio of PS-IR in the Tau-positive axons around the Pyr-layer of the CA1 region after injection of KA or saline. The ratio of PS-IR was significantly higher after KA injection compared with controls injected with saline (**p<0.01). Bars = 20 µm (a, b) and 10 µm (1R–6M, c–f).