Prosaposin Overexpression following Kainic Acid-Induced Neurotoxicity
Figure 3
a–y: In situ hybridization of the hippocampus showing PS mRNA after saline injection (a–e), or 1 (f–j), 3 (k–o), 7 (p–t) and 21 days (u–y) after KA injection into the CA1 (b, g, l, q, v), CA3 (c, h, m, r, w), CA4 (d, i, n, s, x), and dentate gyrus (DG, e, j, o, t, y).
PS mRNA expression increased after KA injection compared with saline injection. Bar = 1,000 µm (a, f, k, p, u) and 100 µm (b–e, g–j, l–o, q–t, v–y). z: The ratio of the PS mRNA signals in the hippocampal CA1, CA3, CA4, and DG regions after the injection of KA or saline. The intensity of PS mRNA, semi-quantified as the ratio of reaction products, significantly increased on days 1, 3, 7 and 21 after KA injection compared with saline injection. The data are presented as means ± standard error (S.E.). **p<0.01; significantly different from control.