Stimulators of Mineralization Limit the Invasive Phenotype of Human Osteosarcoma Cells by a Mechanism Involving Impaired Invadopodia Formation
Figure 8
Invadopodia formation and proteolytic activity in 143B cells treated with ascorbic acid and β-glycerophosphate.
143B cells were cultured in control conditions or in the presence of AA/B-GP for 24 h. (A) Determination of the number of invadopodia per cell. Invadopodia of cells that had assembled at least one invadopodium were counted in 10 visual fields. Data are expressed as means values ± SEM from multiple experiments, *p≤0.05 by Student’s t-test. Panel on the left shows representative confocal images of invadopodia (counterstained for cortactin and F-actin) in 143B cells. Reconstruction of the Z-cut section profile is shown. Scale bar = 20 µm. (B) Analysis of fluorescent matrix degradation normalized to total area of the cells. Data are expressed as means ± SEM from multiple experiments, *p≤0.05 by Student’s t-test. Typical images of Oregon green-488 labeled gelatin (left panels), phalloidin-TRITC stained F-actin (middle panels) and merged images (right panel) are presented. Scale bar = 50 µm.