Functional Phenotype of Synovial Monocytes Modulating Inflammatory T-Cell Responses in Rheumatoid Arthritis (RA)
Figure 1
Frequency of CD14+CD16+, but not CD14dimCD16+, monocytes is increased in peripheral blood of rheumatoid arthritis (RA) patients compared with healthy controls (HC).
(A) Representative contour plot of peripheral monocytes from HC (left) and RA patients (right). Monocytes were defined as cells expressing HLA-DR but not expressing any lineage markers, such as CD3 (T cells), CD19 (B cells), or CD56 (NK cells). Gated cells were subdivided into three monocyte subsets by their expression of CD14 and CD16. Number in rectangular gate indicates the frequency of CD14dimCD16+ monocytes. (B) Frequencies (%) of three monocyte subsets in peripheral blood from HC (n = 14) and RA patients (n = 23) (C) Frequencies (%) of monocytes expressing CD14 and HLA-DR among peripheral blood mononuclear cells (PBMC) between HC and RA patients. (D) The frequency of peripheral CD14+CD16+ monocyte was plotted against serum C-reactive protein levels (CRP) among RA patients (n = 22). Each data point represents an individual subject; horizontal bars and error bars show the mean ± SEM. * = P<0.05 by unpaired t-test (B). P value in (D) was obtained using the Pearson correlation analysis.