TGFβ-Induced Deptor Suppression Recruits mTORC1 and Not mTORC2 to Enhance Collagen I (α2) Gene Expression
Figure 5
TGFβ-induced Hif1α expression is translationally regulated by deptor expression.
Human proximal tubular epithelial cells were cotransfected with the Hif1α 5′UTR-fused Renilla luciferase and FLAG-Deptor (panel A) or deptor shRNAs (panel B). The transfected cells were treated with TGFβ for 24 hours. The cell lysates were used to assay Renilla luciferase activity as described [5], [39]. In panel A, Mean ± SE of 5 measurements is shown. *p<0.001 vs control; **p<0.001 vs TGFβ-stimulated. In panel B, Mean ± SE of 3 measurements is shown. *p<0.05 vs control. Expression of deptor for these panels from parallel samples is shown in Fig. S9.