Reciprocal Regulation of Epileptiform Neuronal Oscillations and Electrical Synapses in the Rat Hippocampus
Figure 5
Cx36 expression in parvalbumin (PV)-positive interneurons into CA3.
In order to quantify the amount of Cx36 (green) in PV-positive cells (red), we performed double-labeling experiments in coronal sections of rats from control, acute and latent groups counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue). (A–D) High magnification of the selected area highlighted in the control section (E) shows Cx36 (A), PV (B) and DAPI labeling (C). Colocalization of both proteins is evidenced in (D). (E, F, G) In representative images of CA3 area it is possible to visualize that Cx36 remains in PV-positive cells in acute (F) and latent (G) groups. The pixel intensity profile revealed intersection of the green and red signals, supporting the colocalization of both proteins. (H) Pixel intensity analysis showed no significant differences in Cx36 mean intensity in PV-positive cells between the groups. (I–K) Intensity correlation between green (Cx36) vs. red (PV) channels of representative images from control, acute and latent periods, respectively. (L) Quantitative analysis (n = 4 animals per group) revealed that Mander's colocalization coefficient did not change in acute and latent groups. Bars represent standard errors of mean. Scale bar: 50 µm.