ZFC3H1, a Zinc Finger Protein, Modulates IL-8 Transcription by Binding with Celastramycin A, a Potential Immune Suppressor
Figure 5
Effect of Celastramycin A on HeLa cells.
(A) IL-8 expression levels in HeLa cells transfected with control or ZFC3H1 siRNA. RNAs were isolated from HeLa cells 5 h after UV (20 J/m2) irradiation. The concentration of Celastramycin A was 0.05 µg/ml. Data were normalized to β-actin and control HeLa data was set as 1. Three independent experiments. (B) IL-8 expression levels in HeLa cells with control shRNA (left) and shERCC1 (right). RNAs were isolated from HeLa cells 5 h after UV (20 J/m2) irradiation. The concentration of Celastramycin A was 0.05 µg/ml. Data were normalized to β-actin and control HeLa (shRNA-control, left) or HeLa (shRNA-ERCC1, right) data was set as 1. Three independent experiments. (C) Confocal microscopic images showing the localization of ZFC3H1 (green) and ERCC1 (red). Scale bar, 5 µm. *P<0.05. NS, not significant.