Immunogenicity Evaluation of a Rationally Designed Polytope Construct Encoding HLA-A*0201 Restricted Epitopes Derived from Leishmania major Related Proteins in HLA-A2/DR1 Transgenic Mice: Steps toward Polytope Vaccine
Figure 8
Cytotocic T cell response against RMA/s target cells loaded with individual peptides.
Cytolytic activity was tested in a standard 4-hour 51Cr release assay. RMA/s target cells loaded with individual peptides at a 10 µg/ml final concentration and labeled with 51Cr radioactive isotope were co-cultured with short term CTL lines making three different effector-to-target ratios. Results are expressed as the mean of triplicates in % of specific lyses: [(experimental − spontaneous release)/(total – spontaneous release)] ×100. A 9-mer HLA-A0201 restricted peptide from human telomerase was used as negative control.