Complex Impacts of PI3K/AKT Inhibitors to Androgen Receptor Gene Expression in Prostate Cancer Cells
Figure 6
LY294002 and AZD5363 affect AR and AR-V7 transcriptional activity in PCa cells.
(A) LNCaP and (B) VCaP cells were transfected with control or AKT1–3 siRNAs for 48 hours and treated with vehicle or 1 nM R1881. Cells were also co-treated with vehicle, 50 uM LY294002 or 5 uM AZD5363 for 18 hours. Real-time PCR assays measured PSA and OPRK1 mRNA levels. (C) LNCaP95 and (D) VCaP cells were transfected with control or AKT1–3 siRNAs for 48 hours and treated with vehicle, 50 uM LY294002 or 5 uM AZD5363 in the presence of 5 uM MDV3100 for 18 hours. Real-time PCR assays measured UGT2b17 mRNA levels. Results were from three independent RNA extractions with triplicate real-time PCR assays on each RNA sample. Student's t-tests were performed comparing between vehicle and LY294002/AZD5363 treatments with * as P<0.05; ** as P<0.01 and *** as P<0.001.