Antisense Oligonucleotides Capable of Promoting Specific Target mRNA Reduction via Competing RNase H1-Dependent and Independent Mechanisms
Figure 8
Effect of HSPA8 reduction on ASO 440282 activity.
SOD/TO cells were treated with siRNA to reduce expression of HSPA8. After 48 hours, cells were transfected with ASOs at concentrations from 10 to 100 nM. Following a 4-hour transfection, minigene expression was induced by the addition of 1 µg/ml TET for 3 hours. A) Western blot of HSPA8 at 48 hours post-siRNA treatment. B) Levels of spliced mRNA relative to levels in mock-treated cells. Black lines = control cells, grey lines = HSPA8-deficient cells.