Role of the Exogenous HCV Core Protein in the Interaction of Human Hepatocyte Proliferation and Macrophage Sub-Populations
Figure 6
p-STAT3 and CD206 is upregulated in mTHP-1 cells.
A. mTHP-1 cells co-cultured with L02 cells by transwell co-culture system in the presence of exogenous HCV core protein (10 µg/ml) or unrelated protein (GST, GAS/M protein), at indicated time-points the mTHP-1 was harvested to perform Western blot. (a) In the presence of the exogenous HCV core protein at 4 h; (b) In the presence of respective GST, GAS/M, inactive-GST, inactive-GAS/M protein at 4 h; (c) In the presence of exogenous HCV core protein (10 µg/ml) at different time-points (0, 2, 4, 6, 8, 10 h). B. mTHP-1 cells were co-cultured with L02 cells and the exogenous HCV core protein (10 µg/ml) for 24 h, then stained with APC-labeled CD206 specific antibody. The percentage of CD206-positive cells was analyzed by flow cytometry (showed in the upper-right corner). (a) mTHP-1 cells (PBS control group); (b) mTHP-1 cells were co-cultured with L02 cells; (c) mTHP-1 cells were co-cultured with L02 cells and the exogenous HCV core protein; (d) mTHP-1 cells were treated with the exogenous HCV core protein.