EGF Up-Regulates miR-31 through the C/EBPβ Signal Cascade in Oral Carcinoma
Figure 5
Curcumin down-regulates miR-31 expression via EGF downstream signals in OSCC cells.
(A) Western blot analysis. (B, C) qRT-PCR analysis. (A) SAS cells. (B, C) SAS cells (Upper) and HSC-3 cells (Lower). (A) The analysis shows the attenuation of EGF induced AKT activation and C/EBPβ up-regulation after treatment with 12 µM curcumin for 24 h. EGF induced ERK activation was not obviously attenuated by curcumin. Treatment with EGF or curcumin had little effect on the expression of β-catenin or Bcl2. (B) Curcumin treatment down-regulates endogenous C/EBPβ mRNA expression and EGF induced C/EBPβ mRNA expression in SAS cells and HSC-3 cells. (C) Curcumin attenuates endogenous miR-31 expression and EGF induced miR-31 expression in both types of cell. The numbers below the pictures are normalized values. #, quantification unavailable due to faint image signals. Data are the means ± SE from at least triplicate analysis. *, p<0.05; **, p<0.01; ***, p<0.001; un-paired t-test.