FLASH/casp8ap2 Is Indispensable for Early Embryogenesis but Dispensable for Proliferation and Differentiation of ES Cells
Figure 3
(A) Genome structures of WT and FLASH mutant mice. Arrows (number 5-8) indicate the position of the primers for genomic PCR, and the black box (Neo probe) indicates the position of the probe for Southern blot analysis. LTR; viral long terminal repeat, SA: splice acceptor, SV40tpa: SV40 poly adenylation sequence, SD: splice donor. (B) Genomic PCR analysis showed that the trapping vector was inserted between exons 1 and 2 of the mouse FLASH gene in WT and two FLASHmut/+ (mut/+1 and mut/+2) mice. (C) Southern blot analysis of WT and FLASHmut/+ mice. Genomic DNA from the tail tips was digested by EcoRΙ and hybridized with the Neo probe. The 3123 bp fragment showed the trapping vector inserted into a mutant allele.