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Autophagy and Apoptosis in Hepatocellular Carcinoma Induced by EF25-(GSH)2: A Novel Curcumin Analog

Figure 4

Morphology of autophagosomes in EF25-(GSH)2-treated HepG2 cells.

HepG2 cells were treated with 20 µmol/L EF25-(GSH)2 for 16 h and observed under transmission electron microscopy. (A) and (B), multimembranous autophagic vacuoles engulfing cytoplasmic components are indicated with black arrowheads. (C) and (D), autophagic vacuoles containing a mitochondrion are indicated with black asterisk.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0107876.g004