Regression of Glomerular and Tubulointerstitial Injuries by Dietary Salt Reduction with Combination Therapy of Angiotensin II Receptor Blocker and Calcium Channel Blocker in Dahl Salt-Sensitive Rats
Figure 7
NADPH oxidase dependent superoxide anion production in renal tissue in Protocol 2.
A, Representative images of 4-hydroxy-2-nonenal (4-HNE) immunostaining (scale bar shows the values). B, Quantitative analysis of 4-HNE-positive area. C, NADPH oxidase activity in homogenized renal cortical tissues measured by lucigenin-enhanced chemiluminescence. Renal cortical tissue (D) gp91phox and (E) Nox-1 mRNA levels were determined by real time RT-PCR. β-actin was used as an internal control and results are expressed as fold changes to 10-week values of the DSS + NS group. #P<0.05 vs. 10-week values of DSS + HS group. aP<0.05 vs. DSS + NS, bP<0.05 vs. DSS + HS, cP<0.05 vs. DSS + HS → NS, dP<0.05 vs. DSS + HS → NS + OLM or DSS + HS → NS + AZEL.