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Genome Analysis of Environmental and Clinical P. aeruginosa Isolates from Sequence Type-1146

Figure 7

The CRISPR-Cas system detected in the ST-1146 isolates.

The system was constituted by the following elements: CRISPR2, contains 29 spacers in the environmental isolates and 30 in SD9 (additional spacer indicated by*); Cas1 (cas1) endonuclease; nuclease/helicase Cas3 (cas3); 3 associated proteins Csy1 (csy1), Csy2 (csy2) and Csy3 (csy3) and a CRISPR-associated endonuclease Cas6/Csy4 encoded by the cas6f gene. The spacers (32 bp) are represented by gray rectangles between black triangles (repeats of 28 bp). The additional spacer found in the CRISPR2 of SD9 is labelled by an asterisk. Two potential leader sequences (L) of up to 244 bp are represented in front of both CRISPR loci.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0107754.g007