New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca2+ Handling in Differentiated Satellite Cells
Figure 4
Purine-induced intracellular Ca2+ rises in myoblasts.
(a–d) Representative intracellular Ca2+ variations in the wild-type (WT) and MLC/mIGF-1 transgenic (TG) myoblasts, expressed as fluorescence ratios (340/380). The time courses were recorded during the addition of ATP (a, c) and GTP (b, d). (e) Quantification of intracellular Ca2+ response parameters determined for the wild-type and MLC/mIGF-1 transgenic myoblasts. They include: the percentage of cells responsive to each stimulus, the Velocity to reach the peak of Ca2+ increase, calculated as amplitude to time to peak (v = amplitude s−1); Maximum amplitude calculated as the ratio of the F340/380 at the peak to the basal F340/380; Amplitude at 1 min stimulation calculated as the ratio of the F340/380 at 1 min from application of stimulus to the basal F340/380. * p<0.05; *** p<0.001.