Sheep Lung Segmental Delivery Strategy Demonstrates Adenovirus Priming of Local Lung Responses to Bacterial LPS and the Role of Elafin as a Response Modulator
Figure 5
Systemic haematological responses to intrapulmonary adenoviral vector and LPS administration.
Heparinised blood was collected by venepuncture at various time points throughout the experimental period (Pre-treatment (immediately prior to collection of baseline BALF samples), d10 and d10+6h). This blood was analysed for total white blood cell numbers, neutrophil numbers and lymphocyte numbers as indicated in a, b and c respectively. The boxplot tails reflect the range of data, the bottom and tops of the boxes the 25th and 75th percentiles respectively, and the thick horizontal line the median values. A linear mixed-effect model, where sheep-specific responses were assumed a random effect, was used to analyse the influence of Ad-o-elafin and Ad-GFP and subsequent LPS instillation on blood parameters. *, ** and *** represent P<0.05 and P<0.01 and P<0.001 respectively.