1H–NMR Metabolomic Biomarkers of Poor Outcome after Hemorrhagic Shock are Absent in Hibernators
Figure 1
Hydrophilic metabolic profiles.
A: Hydrophilic metabolic profiles for naïve summer euthermic and winter IBA-AGS tended to form distinct clusters separate from each other and the naïve rats. However, there was no statistically significant discrimination between the classes (R2 = 1.00, Q2 = 0.79, permutation p = 0.70) B: I/R injury–prone rats displayed different hydrophilic metabolic profiles before and three hours after hemorrhagic shock (R2 = 0.95, Q2 = 0.79, permutation p<0.01.). No such clustering was evident for the AGS in either hibernation season. PLS-DA analysis of 1H–NMR of naïve plasma samples (A) or samples from before and after hemorrhagic shock (B).