Knock-Down of IL-1Ra in Obese Mice Decreases Liver Inflammation and Improves Insulin Sensitivity
Figure 1
Antisense oligonucleotide (ASO) mediated knock-down of IL-1Ra transcript in vitro and in vivo using locked nucleic acids (LNAs).
A) QPCR analysis of IL-1Ra mRNA expression after ASO treatment in Hepa 1–6 cells, each concentration was run in duplicate. B) QPCR analysis of IL-1Ra and IL-1β mRNA levels in liver and epididymal adipose (eWAT) from mice one week after IL-1Ra ASO administration. C) Blood glucose concentration in mice one week after IL-1Ra ASO treatment. D. QPCR analysis of IL-1Ra mRNA expression in liver and eWAT after 6 weeks of treatment of IL-1Ra ASO or control ASO. E. Plasma IL-1Ra concentration in obese mice treated with IL-1Ra ASO or control ASO for 6 weeks compared with aged matched lean control mice fed normal chow (NC). F. Analysis of liver enzyme levels after IL-1Ra ASO treatment in HFD-fed mice (ALT, alanine aminotransferase; AST, aspartate aminotransferase; GDH, glutamate dehydrogenase). *P<0.05 compared with control treated mice, QPCR results are normalized to beta actin mRNA and expressed relative to control treatment. Differences between groups were analyzed by one way ANOVA followed by Tukey post hoc tests, n = 5–8 mice per group, * p<0.05 compared to HFD-fed control treated group; ns, not significant.