The BiP Cochaperone ERdj4 Is Required for B Cell Development and Function
Figure 2
The loss of ERdj4 impairs B cell development beginning at the large pre-B cell stage.
(A) Representative contour plots from flow cytometric analyses of B cell development using the Hardy classification scheme. Numbers indicate frequency of the gated population for each genotype. Upper panel, gated on IgM− bone marrow cells. Middle panel, gated on B220+CD43+ bone marrow cells. Lower panel, gated on total bone marrow cells. Fraction A, B220+CD43+BP-1−HSA−; Fraction B, B220+CD43+BP-1−HSA+; Fraction C, B220+CD43+BP-1+HSAlow; Fraction C’, B220+CD43+BP-1+HSAhigh; Fraction D, IgM−B220+CD43−; Fraction E, B220lowIgM+; Fraction F, B220highIgM+. (B–C) Absolute numbers of Hardy fractions. n = 5–8 mice/genotype.