Targeting Apoptosis Signalling Kinase-1 (ASK-1) Does Not Prevent the Development of Neuropathy in Streptozotocin-Induced Diabetic Mice
Figure 4
Characterisation of ASK1n transgenic mice.
(A) The ASK1 sequence amplified from control mice cannot be digested with Nhe-1, whilst the sequence amplified from ASK1n can, confirming the presence of the modified ASK1 locus and the introduced Nhe-1 restriction site. (B) Chromatograph of the sequence flanking the mutation site of ASK1n mice, showing single G base substitution in homozygous mice and conversion of lysine (K) in control mice to arginine (R) in ASK1n mice. (C) Coomassie staining demonstrates that equal amounts of MBP substrate were added to each kinase reaction. (D) An immune complex kinase assay confirms phenotypic loss of kinase activity in brain tissue from ASK1n mice compared to control C57 mice (D, E **p<0.001; Students unpaired t-test).