A Novel Binary T-Vector with the GFP Reporter Gene for Promoter Characterization
Figure 5
Detail analysis in the expression patterns of the gene LOC_Os10g35930.
(A) The expression profile of the gene shown by the MPSS dataset. Samples were labeled as below: 1, 3-day old germinating seeds; 2, 10 days of germinating seedlings; 3, 14-day old young leaves; 4, 14day old young roots; 5, 60-day old mature leaves; 6, 60-day old mature roots; 7, 60-day old stems; 8, immature panicles; 9, mature pollens; 10, merismatic tissues; 11, ovary and mature stigmas; 12, rice developing seeds. (B) The gene showed mature inflorescence specific expression according to the microarray dataset with the GEO accession number GSE6893. Samples were labelled as below: 1, 7-day old roots; 2, mature leaves; 3, young leaves; 4, shoot apical meristem; 5, 0–3 cm inflorescence; 6, 3–5 cm inflorescence; 7, 5–10 inflorescence; 8, 10–15 cm inflorescence; 9, 15–22 cm inflorescence; 10, 22–30 cm inflorescence; 11, 0–2 day old seeds, 12, 3–4 day old seeds; 13, 5–10 day old seeds; 14, 11–20 day old seeds; 15, 21–29 day old seeds. (C) The gene showed the highest expression at mature pollens by the microarray dataset with the GEO accession number GSE17002. Samples were labelled as below: 1, seedling; 2, pollens at anthesis; 3, sperms at anthesis. (D) The gene showed the highest expression level at mature and germinated pollen grains. Samples were labelled as below: 1, callus cells; 2, leaves; 3, roots; 4, uninucleate microspores; 5, bicellular pollens; 6, tricellular pollens; 7, mature pollen grains; 8, germinated pollen grains. (E) The gene showed the anther-specific expression by the RNA_Seq data. The samples were labelled as below: 1, shoots; 2, 20 day old leaves; 3, pre-emergence inflorescence; 4, post-emergence inflorescence; 5, anthers; 6, pistil; 7, 5 day old seeds; 8, 10 day old seeds; 9, 25 day old seeds; 10, , 25 day old endosperms. (F) The gene showed the highest expression at the booting and flowering panicles by qRT-PCR. Samples were labelled as below: 1, two-week old leaves; 2, two-month old leaves; 3, two-week old roots; 4, two-month old roots; 5, 0–5 cm long panicles; 6, 5–10 cm long panicles; 7, more than 10 cm long panicles; 8, booting panicles; 9, flowering panicles; 10, milky seeds; and 11, mature seeds.