The Development of Diet-Induced Obesity and Glucose Intolerance in C57Bl/6 Mice on a High-Fat Diet Consists of Distinct Phases
Figure 7
A. Plasma protein levels of haptoglobin (P<0.05), alpha-1-antichymotrypsin (aACT) (P<0.01) and ApoA-IV (P<0.001) were all significantly higher in HF compared to LF after 3 days on the diet. B. After 1 week on the diet plasma protein levels of ApoA-IV were significantly greater in HF fed mice compared with LF (P<0.01). There was no significant difference in haptoglobin or aACT protein levels between mice on HF vs. LF diets after 1 week. C. After 16 weeks on the diet there was significantly greater level of haptoglobin protein in plasma from HF compared to LF (P<0.01), there was no significant difference in the levels of ApoA-IV or aACT in HF compared to LF (n = 6–8). D. Alpha-1-antichymotrypsin (aACT) plasma protein levels were significantly greater in HF compared to LF (P<0.01) and PF (P<0.001) at 3 days. There were no significant differences in aACT plasma protein levels after 1 week. E. ApoA-IV plasma protein was significantly increased in HF compared to LF at 3 days (P<0.001). At 1 week ApoA-IV plasma protein levels were higher in HF compared to both LF (P<0.001) and PF (P<0.05). The amount of ApoA-IV protein was also greater in plasma from PF mice compared to LF (P<0.01) after 1 week. F. Levels of haptoglobin protein in plasma from HF mice were significantly greater at 3 days compared to LF (P<0.05). There was no significant difference in plasma protein levels of haptoglobin between the different diets after 1 week (n = 6–8).