Differential Susceptibilities to BmNPV Infection of Two Cell Lines Derived from the Same Silkworm Ovarian Tissues
Figure 2
Comparison of susceptibilities of BmN-SWU1 and BmN-SWU2 ovarian cell lines to BmNPV infection.
(A) Schematic diagram illustrating construction of donor plasmids and generation of recombinant BmNPV vA4prm-VP39-EGFP, which contains the EGFP fluorescence marker gene driven by the silkworm actinA4 promoter. (B) Fluorescence microscopy showing the progression of infection in both cells lines infected with vA4rpm-VP39-EGFP (MOI = 2 or 20) at 24, 48 and 72 h p.i. (C) BmNPV infection efficiencies (EGFP positive, EGFP+) in BmN-SWU1 and BmN-SWU2 cells were determined by counting fluorescent cells in five different fields of view. Three independent experiments were carried out with three replicates each. Data are presented as means ± SD (n = 3). Statistically significant differences: **P<0.01. (D) Western blot analysis of silkworm ovarian cells lysed at indicated times after vA4rpm-VP39-EGFP infection (MOI = 20) using an α-EGFP antibody.