Relationships between Signaling Pathway Usage and Sensitivity to a Pathway Inhibitor: Examination of Trametinib Responses in Cultured Breast Cancer Lines
Figure 6
The effects of drug combinations on MCF-7, T47D and SKBr3 cell lines.
Left hand panels: Growth inhibitory effects of combinations of trametinib with everolimus (TraEvl), NVP-BEZ235 (TraBEZ) and GSK2126458 (TraGSK) using the Chou-Talalay method. The combination index (CI) theorem of Chou-Talalay defines Synergism: CI<0.9; additivity: 0.90≤CI≤1.1; antagonism: 1.1≤CI≤10 in drug combinations. X axis, FA, the ratio of the fraction affected; y axis, CI, the combination index. Results were from two independent experiments. Right hand panel: Drug combination effect on phosphorylation of ERK, AKT, p70S6K and rpS6 in (A) MCF-7, (B) T47D, (C) SKBr3 cell lines after 24 h as demonstrated by immunoblotting with anti-phospho antibodies. Tubulin is the loading control.