Characterization of the Regulatory Mechanisms of Activating Transcription Factor 3 by Hypertrophic Stimuli in Rat Cardiomyocytes
Figure 8
In vivoATF3 overexpression downregulates the gene expression of PAI-1.
Three days following the adenoviral injection of ATF3 or LacZ (1×109 pfu) into the left ventricular wall of adult rats, the animals were sacrificed and mRNA was extracted and cDNA synthesized for RT-qPCR analysis. ATF3 (A), ANP, BNP, α- and ß-MHC, cardiac and skeletal α-actins (B), PAI-1 (C) and IL-6 (D) mRNA levels were normalized to 18S quantified from the same samples and presented relative to LacZ-injected animals. The results are mean ± SEM (n = 4–5). *P<0.05; **P<0.01.