ALKBH4 Depletion in Mice Leads to Spermatogenic Defects
Figure 4
Pachytene spermatocytes in Alkbh4Δ/Δ mice fail to maintain synaptonemal complex.
Upper panels, immunofluorescence labeling of histological sections for SYCP3 (red) and DAPI (blue counterstain) allows distinguishing leptotene (L) spermatocytes, cells at leptonema-zygonema transition (L/Z), as well as pachytene (P) and diakinesis-stage (D) spermatocytes by the characteristic development of the synaptonemal complex (SC). Lower panels, among pachytene spermatocytes in Alkbh4Δ/Δ mice, cells with disordered SC can be observed (arrows). Scale bars, 10 µm.