An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid
Figure 4
Size exclusion chromatography of sheep HCF.
A. Overlay of five HCF chromatograms showing the highly reproducible profile achievable despite the heterogeneity of the starting samples. Aliquots of sheep HCF were desalted and loaded on a Superdex-200 column. Size calibration marks, obtained from runs of standard proteins (thyroglobulin, apoferritin, beta-amylase, BSA and carbonic anhydrase), are indicated by arrows in the chromatogram. B and C: the numbered peak fractions (1–4) were collected and analyzed by western immunoblotting, after SDS-PAGE on 4–10% acrylamide gels, under non reducing and reducing (r) conditions. Two patient sera (serum 1, panel B, and serum 2, panel C, respectively) were tested against all the fractions. Arrows indicate the position of Ag5 and AgB.