7,8,4′-Trihydroxyisoflavone Attenuates DNCB-Induced Atopic Dermatitis-Like Symptoms in NC/Nga Mice
Figure 1
NC/Nga mice were divided into five groups: (1) naïve control (vehicle), (2) DNCB+vehicle, (3) DNCB+200 nmol 7,8,4′-THIF, (4) DNCB+400 nmol 7,8,4′-THIF, and (5) DNCB+tacrolimus. To induce AD-like symptoms, DNCB was topically applied on NC/Nga mice. A day after complete dorsal hair removal, 1% DNCB was applied (day −4). Five days after dorsal hair removal, 0.2% DNCB was challenged three times a week for three weeks (day 0–20). 7,8,4′-THIF or tacrolimus was topically applied seven times a week for 3 weeks (day 0–20).