Self-Assembly and Release of Peste des Petits Ruminants Virus-Like Particles in an Insect Cell-Baculovirus System and Their Immunogenicity in Mice and Goats
Figure 4
Experimental schedule and peste des petitsruminants virus (PPRV)-specific IgG antibody responses to PPRV-H or PPRV-F virus-like particles (VLPs).
Groups of mice were immunized subcutaneously, twice, as indicated, with 4-week intervals. Three mice from each group were sacrificed and splenocytes were pooled for the lymphocyte proliferation assay. Enzyme-linked immunosorbent assay (ELISA) plates were coated with whole PPRV, as indicated in the Materials and Methods section. Serially diluted sera were used after primary and booster vaccinations. Total immunoglobulin G levels were determined from mice immunized with PPRV VLPs or PPRVNig75/1.