Toll-Like Receptor-3 Is Dispensable for the Innate MicroRNA Response to West Nile Virus (WNV)
Figure 3
WNV infection induces a unique NFκB-responsive gene expression profile.
HEK293-Null and –TLR3 cells under the specified conditions were analyzed for gene expression profiles using a qPCR array consisting of ∼90 NFκB-responsive genes. (A,B) Fold induction of genes was calculated by comparing WNV-infected samples to cells treated with formalin-inactivated virus. WNV-induced gene expression is shown at 0 hours post-infection (A) and 2 hours post-infection (B) with WNV (MOI = 10). Black bars denote TLR3-expressing HEK293 cells while open bars represent HEK293-Nulls. (C) The median CT across all samples was calculated and is shown +/− SEM for each gene analyzed. All genes shown were abundantly expressed (CT<35). (D) Heatmap of NFκB genes profiled. Highly abundant miRNAs are indicated by red and non-expressed miRNAs by blue color. Yellow indicates an intermediate expression level. This is also shown underneath the heatmap. The miRNAs were clustered based on expression.