Serotonin 5-HT3 Receptor-Mediated Vomiting Occurs via the Activation of Ca2+/CaMKII-Dependent ERK1/2 Signaling in the Least Shrew (Cryptotis parva)
Figure 8
Suppressive effects of ERK inhibition on 5-HT3R-mediated emesis.
A) The cited doses of the ERK inhibitor PD98509 were administered to different groups of shrews 30 min prior to 2-Me-5-HT (5 mg/kg, i.p.) injection. The vomit parameters were recorded for 30 min post 2-Me-5-HT injection. The vomit frequency data are presented as mean ± SEM. **P<0.01 and ***P<0.001 vs. vehicle-pretreated control. B) PD98059 (5 mg/kg, i.p.) or its vehicle (i.p.) was administered to different groups of shrews 30 min prior to 2-Me-5-HT (5 mg/kg, i.p.) injection and immunoblot analyses of ERK1/2 phosphorylation were performed on shrew brainstems collected 10 min after 2-Me-5-HT treatment. n = 3 per group. Graph B shows the summarized data and the insets show the representative Western blot. *P<0.05 vs. control vehicle/vehicle, #P<0.05 vs. Vehicle + 2-Me-5-HT.