Inhibition of CSF-1R Supports T-Cell Mediated Melanoma Therapy
Figure 1
Systemic inhibition of CSF-1R decreases B16F10-induced accumulation of blood monocyte subsets.
(A) Myeloid populations in the blood were monitored by flow cytometry following B16F10 tumor growth. Percentages of CD11b+ myeloid cells out of total white blood cells (WBC) and frequencies of CD11b+F4/80+CSF-1R+ monocytes or CD11b+Gr-1high granulocytic cells within the CD11b+ population are depicted of control- and PLX3397-treated mice. Data are compiled from two different experiments with ten mice per group. Linear regression analysis indicated a significant difference in frequencies between control- and PLX3397-treatment measurements for all three graphs and a significant slope difference of CD11b+F4/80+CSF-1R+ cells (p = 0.04). (B) Frequencies of CD11b+F4/80+ monocytes in the blood as a proportion of total white blood cells at day 13 of B16F10 tumor growth. (C) Flow cytometry plots of blood CD11b+F4/80+ cells at day 13 of B16F10 tumor growth. (D) Frequencies of Ly6C- cells within this gate are plotted for eight mice per group. Means and standard error of the mean of five mice per group are depicted from one out of two similar experiments.