Improved Efficacy and Reduced Toxicity of Doxorubicin Encapsulated in Sulfatide-Containing Nanoliposome in a Glioma Model
Figure 3
Intracellular retention of SCN-DOX in U-118MG cells.
U-118MG cells were first incubated with 2 µg/mL free DOX or equivalent SCN-DOX for 24 hours. Cells were then washed with PBS twice and cultured in fresh full culture medium. The same wells of cells were imaged serially at 1 h, 2 h, 4 h and 24 h after washing using fluorescence confocal microscopy. (A) Cells treated with free DOX. (B) Cells treated with SLC-DOX. Data are typical of three independent experiments. Red: fluorescence from DOX; blue: nuclei stained with Hoechst 33342. Scale bars: 20 µm.