Arsenic Trioxide Reactivates Proteasome-Dependent Degradation of Mutant p53 Protein in Cancer Cells in Part via Enhanced Expression of Pirh2 E3 Ligase
Figure 4
Knockdown of Pirh2 inhibits arsenic-induced degradation of mutant p53 protein.
Western blots were prepared with extracts from HaCaT (A) and MIA PaCa-2 (B) cells, which were transfected with scrambled siRNA #1 (Scr-1) (lanes 1–2), Scr-2 (lanes 5–6), siRNA against Pirh2-1 (siPirh2-1) (lanes 3–4), or siPirh2-2 (lanes 7–8), and then treated with ATO for 6 h. The blots were then probed with antibodies against Pirh2, p53, and actin, respectively.