NAC Attenuates LPS-Induced Toxicity in Aspirin-Sensitized Mouse Macrophages via Suppression of Oxidative Stress and Mitochondrial Dysfunction
Figure 3
A–D: LPS- and ASA-induced alterations in GST and GSH Px activities.
J774.2 macrophage cells were cultured to 80% confluence and treated with LPS alone or in combination with ASA with or without NAC as described in Materials and Methods. GST-dependent GSH conjugation (3A–3B) and GSH-Px (3C–3D) activities were measured as described before [13]. Results are expressed as mean +/− SEM of at least three experiments. Asterisks indicate significant difference (*p≤0.05, **p≤0.001 from control (C) and δ p≤0.05 compared to LPS and ASA treated cells.