Metagenomic Identification of a Novel Salt Tolerance Gene from the Human Gut Microbiome Which Encodes a Membrane Protein with Homology to a brp/blh-Family β-Carotene 15,15′-Monooxygenase
Figure 6
EZTn5 transposon mutagenesis of SMG 6 was performed to identify mutants lacking pigmentation when grown in the presence of β-carotene.
(A) Clones positive for a transposon in this region of SMG 6 fosmid insert were identified by PCR, with amplicons of ∼3.3 kb indicative of an insertion event. (B) Approximate locations of transposon insertions in relation to brpA and neighbouring genes. (C) Appearance of cell pellets of SMG 6 and transposon insertion mutants (EZTn #24, #26, #34 and #38) following growth in the presence of β-carotene.