An Abp1-Dependent Route of Endocytosis Functions when the Classical Endocytic Pathway in Yeast Is Inhibited
Figure 5
The Effect of Abp1 mutations on Bulk Endocytosis.
(A) Schematic of full length Abp1 and the different mutants analysed in this study. (B) Wild type, abp1Δ and abp1Δ cells expressing wild type ABP1 or mutant versions were grown to mid log phase before treating with Lat-A and following uptake of FM4-64. Cells were classified according to the most prominent staining after 20 minutes FM4-64 incubation. Error bars are standard deviation. (C) The effect of Abp1 mutations on actin organization as assessed by rhodamine phalloidin staining. (D) A number of proteins have been shown to bind to the SH3 domain of Abp1. Deletions in genes encoding 3 of these proteins, Ark1, Prk1 and Srv2/CAP were analysed to determine whether any of these interactions is potentially required for the Abp1 dependent endocytic pathway in the presence of 25 µM Lat-A. Cells were classified according to the most prominent staining after 20 minutes FM4-64 incubation. Error is Std Deviation.