Co-Expression and Co-Purification of Archaeal and Eukaryal Box C/D RNPs
Figure 5
Quantitative RT-PCR analysis of RNA samples from co-expressing box C/D RNPs.
Delta Rn indicates the magnitude of amplification and is the normalized and base-line corrected fluorescence emission intensity of the reporter dye obtained for each PCR reaction. A). Amplification plots of qRT-PCR of RNA samples extracted from Ni-NTA purified (red) or cell lysate (green) of Archaeoglobus fulgidus sR3 sRNP. B). Amplification plots of qRT-PCR of RNA samples extracted from Ni-NTA purified (red) or cell lysate (green) of human U14 snoRNP.