Calcium Dependence of Eugenol Tolerance and Toxicity in Saccharomyces cerevisiae
Figure 3
Extracellular Ca2+ is necessary for eugenol tolerance.
Yeast cultures were spotted onto YPD, YPD supplemented with 102 agar plates containing either 0 (1% ethanol), 1.3, 2.6 or 3.2 mM eugenol. Left-most spots on each plate are growth after 2 days at 30 C after inoculation with 5 µl culture at approximately 0.5×108 cells/ml. Serial 10-fold serial dilution of the left-most inoculum is shown to the right.