MUC1 Positive, Kras and Pten Driven Mouse Gynecologic Tumors Replicate Human Tumors and Vary in Survival and Nuclear Grade Based on Anatomical Location
Figure 5
Kras- and Pten- induced tumors differ in nuclear grade and survival based on the anatomical site of mutation activation.
(A) Nuclear grade of primary tumor tissues of the ovary, oviduct and the uterus. Representative H&E images are shown. Scale bars: Main −20 µm, Inset −100 µm. (B) Kaplan Meyer curve shows that mice with uterine tumors survive significantly more than those with ovarian tumors (* p = 0.0015) or those with ductal tumors (# p = 0.0016). Individual group comparison after post ANOVA Bonferroni correction (p<0.016). The numbers of mice in each tumor group and median survival time for each tumor type are listed Table S1. Mice with premalignant lesions in the uterine tumor category were excluded from analyses. (C) Splenic Treg/CD8 T cell ratios in mice with ovarian, oviduct or uterine tumors (n = 5 mice/group), represented as box and whisker diagrams (min, Q1, median, Q3, max). CD4 and CD8 T lymphocytes were gated under the CD3 population. Foxp3 cells were gated under the CD4 population. One way ANOVA for comparison of all means (p<0.03) and two tail t tests between any two groups show significant differences between the ratios in uterine tumors and any of the other two tumor types, ovarian and oviduct (p<0.02 and p<0.01, respectively).