Adipose Tissue-Derived Mesenchymal Stem Cells in Long-Term Dialysis Patients Display Downregulation of PCAF Expression and Poor Angiogenesis Activation
Figure 2
Differentiation capacities of HC-MSCs and KD-MSCs.
(A) Adipogenic differentiation of HC-MSCs (top and left) and KD-MSCs (top and right) was examined after 2 weeks of culture under adipogenic conditions by Sudan III staining (original magnification, ×100). Osteogenic differentiation of HC-MSCs (second from top and left) and KD-MSCs (second from top and right) was examined after 4 weeks of culture under osteogenic conditions by von Kossa staining (original magnification, ×100). Chondrogenic differentiation of HC-MSCs (bottom and left) and KD-MSCs (bottom and right) was examined after 3 weeks of culture under chondrogenic conditions by Safranin O/Fast green staining (original magnification, ×100). (B) GPDH activity of cells was measured to compare the adipogenic differentiation capacities of HC-MSCs (n = 5) and KD-MSCs (n = 5). Data are expressed as the mean ± standard error (SE). *P<0.05. (C) ALP activity of the cells was measured to indicate their osteogenic differentiation capacity (n = 4). Data are expressed as the mean ± SE. *P<0.05.