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Bacteria Hold Their Breath upon Surface Contact as Shown in a Strain of Escherichia coli, Using Dispersed Surfaces and Flow Cytometry Analysis

Figure 4

Single cell adhesion assay.

(A) FL1 (upper graph) and FL3 versus FSC dot plots of a mixed microsystem of 3 µm particles with single free-floating and aggregated bacteria; microsystem prepared at a cell-to-particle ratio equal to 4 and incubated with 5 mM CTC. (B) Microscope image of a single cell-particle conjugate. (C) Histogram of normalized respiration index of the different cell populations comprised in the same sample, measured 40 min after bringing cells and particles into contact.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0102049.g004