Areca Nut Components Affect COX-2, Cyclin B1/cdc25C and Keratin Expression, PGE2 Production in Keratinocyte Is Related to Reactive Oxygen Species, CYP1A1, Src, EGFR and Ras Signaling
Figure 2
Role of cytochrome p450 and HO-1 on ANE-induced cytotoxicity and PGE2 production in GK.
(A) Expression of cytochrome p450 isoforms (1A1, 1A2, 2B6/B7, 2A6/A7, 2C8/19, 2E1, 2F1, 3A3, 3A4, 3A5, 3A7) in cultured GK. One representative RT-PCR picture was shown, (B) Stimulation of CYP1A1 mRNA expression in GK by ANE was noted as revealed by RT-PCR (C) α–naphthoflavone (10 and 25 µM) pretreatment and co-incubation prevented the ANE-induced PGE2 production in GK (n = 10). (D) Effect of α–naphthoflavone on ANE-induced cytotoxicity to GK (n = 7), (E) Effect of ANE on HO-1 gene expression of GK, (F) Effect of catalase on ANE-induced HO-1 gene expression of GK, (G) Effect of Zn-protoporphyrin on ANE-induced PGE2 production in GK. *denotes significant difference when compared with solvent control. #denotes statistical significant difference when compared with ANE (800 µg/ml) solely-treated group (P<0.05).