Anti-miRs Competitively Inhibit microRNAs in Argonaute Complexes
Figure 5
Anti-miRs do not affect the levels of total mature or Argonaute-bound miRNA in vivo.
Total liver RNA isolated from animals 7 days post-subcutaneous administration of anti-miR-122 (10 mg/kg). miRNA levels in total RNA sample, profiled on the Nanostring platform, before (a) and after (b) the glyoxal treatment to separate the miR/anti-miR-122 duplex which interferes with miR-122 detection. c and d) Global miRNA levels, post-glyoxal treatment and profiled on the Nanostring platform, in anti-miR-122-treated animals compared to PBS-treated controls from total RNA (c) or immunopurified Argonaute fractions (Ago) (d). Representative experiments shown from 2 independent experiments (n = 3).