Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS
Figure 3
Difference in the level of globin subunits between erythroid cells differentiated in vitro from adult peripheral blood (PB) CD34+ cells, and from C19, OCE1 and OPM2 CD34+ cells.
PB, C19, OCE1 and OPM2 erythroid cells at day 8 in culture were lysed, proteins subjected to trypsin digest and resultant peptides labeled with isobaric tags for nanoLC-MS/MS based quantitation and comparison. Y-axis represents the fold difference in protein level between erythroid cells differentiated from each iPSC line and adult PB progenitors.