Exposure to Static Magnetic Field Stimulates Quorum Sensing Circuit in Luminescent Vibrio Strains of the Harveyi Clade
Figure 5
Transcript levels of luxA and regulatory genes.
Transcript levels of luxA (A), luxR (B), hfq (C), and VH02969, VH04846, VH05322 and VH05886 genes coding for Quorum Regulatory RNA (Qrr) (D) were measured in SMF-exposed or sham-exposed Vibrio sp. PS1 after 48 or 192 h of growth on nutrient agar 1.5% containing 3% NaCl. Results were normalized to 16S rRNA levels. Transcript levels of sham-exposed bacteria grown for 48 h are assumed equal to 1. Each real-time RT-PCR experiment was repeated three times using RNA samples extracted from replicated bacterial cultures for each treatment, and means and standard deviations (bars) were determined. Asterisks indicate statistically significant differences (p<0.01) between values of SMF-exposed and sham-exposed bacteria at the same time intervals.